The role of RNA structural motifs in RNA-lipid raft interaction.

ABSTRACT: Here, we sought to determine the role of specific RNA structural motifs in the interaction of RNA with model lipid vesicles containing liquid-ordered domains (RAFT liposomes). We show that the presence of several small apical loops within RNA structure favors RNA affinity for RAFT liposomes while the increased number of nucleotides within bulges inhibits this affinity. FRET flow cytometry measurements confirmed a modulation of the interaction of RNA with plasma membrane by the presence of specific RNA structural motifs. The analysis of viral RNA fragments revealed that a long double helix at the apical loop increases the affinity of viral RNA to lipid rafts. The analysis of exosomal Y RNAs secreted by nematode parasites showed that the presence of the EXO-motif GGAG is strongly correlated

SUBMITTER: Manka R 

PROVIDER: S-EPMC11861254 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature