Two sexually compatible monokaryons from a heterokaryotic Lentinula edodes strain respond differently to heat stress.

ABSTRACT:

Background

Despite the extensive research conducted on heat responses of Lentinula edodes heterokaryotic cells, the responses of the two sexually compatible monokaryons to heat stress (HS) remain largely unknown.

Methods

To bridge this gap, we examined the nucleus-specific (SP3 and SP30) heat resistant mechanisms using an integrated physiological, metabolomic and transcriptomic approach.

Results

The results showed that HS elicited the boost of ROS and hampered mycelium growth for both monokaryons. Metabolome and transcriptome analysis demonstrated that the two sexually compatible monokaryons responded differently to HS. For SP3, the differentially expressed genes (DEGs) were significantly enriched in Mitogen-Activated Protein Kinase (MAPK) signaling, cell cycle

SUBMITTER: Guo Y 

PROVIDER: S-EPMC11861359 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature