GORK K⁺ channel structure and gating vital to informing stomatal engineering.

ABSTRACT: The Arabidopsis GORK channel is a major pathway for guard cell K⁺ efflux that facilitates stomatal closure. GORK is an outwardly-rectifying member of the cyclic-nucleotide binding-homology domain (CNBHD) family of K⁺ channels with close homologues in all other angiosperms known to date. Its bioengineering has demonstrated the potential for enhanced carbon assimilation and water use efficiency. Here we identify critical domains through structural and functional analysis, highlighting conformations that reflect long-lived closed and pre-open states of GORK. These conformations are marked by interactions at the cytosolic face of the membrane between so-called voltage-sensor, C-linker and CNBHD domains, the latter relocating across 10 Å below the voltage sensor. The inter

SUBMITTER: Zhang X 

PROVIDER: S-EPMC11861651 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature