The Noncanonical Wnt5a-Ca²⁺ Pathway Mediates Mitochondrial Dysfunction in the Progression of Diabetic Nephropathy via the Mitochondrial Calcium Uniporter.

ABSTRACT: Abnormal Wnt5a expression, mitochondrial abnormalities and calcium overload have been detected in many metabolic diseases. However, the association of Wnt5a-Ca²⁺ and mitochondrial dysfunction in diabetic nephropathy (DN) progression remains unknown. We used streptozotocin-induced DBA2/J male mice as a DN model. The mice were treated with losartan (10 mg/kg/d*12 w) or losartan (10 mg/kg/d*12 w) + levamlodipine (5 mg/kg/d*12 w). High glucose (HG) (40 mmol/L)-induced HK-2 cells were used for in vitro experiments. Wnt5a and mitochondrial calcium uniporter (MCU) expression, mitochondrial dynamics, morphological changes and Ca²⁺ concentration were detected in different groups. Levamlodipine, a kind of calcium channel blocker, in combination with losartan ameliorated tubular

SUBMITTER: Fei Y 

PROVIDER: S-EPMC11862900 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature