Recombinant-Chemosynthetic Biosensors for Probing Cell Surface Signaling of Red Blood Cells and Other Cells.

ABSTRACT: The complex signaling mechanisms in red blood cells (RBCs) enable them to adapt to physiological stresses such as exposure to low O₂ levels, metabolic demands, oxidative stress, and shear stress. Since Ca²⁺ is a crucial determinant of RBC fate, various ion channels, pumps, and exchangers regulate the delicate balance of Ca²⁺ influx and efflux in RBCs. Elevated intracellular Ca²⁺ can activate processes such as membrane phospholipid scrambling and alter RBC deformability, which is essential for effective capillary transit. However, the dynamic information about Ca²⁺ regulation in RBCs is limited. Although static mapping and bioanalytical methods have been utilized, the absence of a nucleus and the presence of hemoglobin create challenges

SUBMITTER: Ubeysinghe S 

PROVIDER: S-EPMC11863169 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature