Comparing Alzheimer's genes in African, European, and Amerindian induced pluripotent stem cell-derived microglia.

ABSTRACT:

Introduction

Genome-wide association studies (GWAS) studies in Alzheimer's disease (AD) demonstrate ancestry-specific loci. Previous studies in the regulatory architecture have only been conducted in Europeans (EUs), thus studies in additional ancestries are needed. Given the prevalence of AD genes expressed in microglia, we initiated our studies in induced pluripotent stem cell (iPSC) -derived microglia.

Methods

We created iPSC-derived microglia from 13 individuals of either high Amerindian (AI), African (AF), or EU global ancestry, including both AD and controls. RNA-seq, ATAC-seq, and pathway analyses were compared between ancestries in both AD and non-AD genes.

Results

Twelve AD genes were differentially expressed genes (DEGs) and/or accessible between ancestries,

SUBMITTER: Moura S 

PROVIDER: S-EPMC11863361 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature