Single-cell multi-omics analysis identifies SPP1⁺ macrophages as key drivers of ferroptosis-mediated fibrosis in ligamentum flavum hypertrophy.

ABSTRACT:

Background

Ligamentum flavum hypertrophy (LFH) is a primary contributor to lumbar spinal stenosis. However, a thorough understanding of the cellular and molecular mechanisms driving LFH fibrotic progression remains incomplete.

Methods

Single-cell RNA sequencing (scRNA-seq) was performed to construct the single-cell map of human ligamentum flavum (LF) samples. An integrated multi-omics approach, encompassing scRNA-seq, bulk RNA sequencing (bulk RNA-seq), and Mendelian randomization (MR), was applied to conduct comprehensive functional analysis. Clinical tissue specimens and animal models were employed to further confirm the multi-omics findings.

Results

ScRNA-seq provided a single-cell level view of the fibrotic microenvironment in LF, revealing significantly increased

SUBMITTER: Fei C 

PROVIDER: S-EPMC11863437 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature