Characterization and genome analysis of novel Klebsiella pneumoniae phage vbKpUKJ_2 isolated from hospital sewage water.

ABSTRACT:

Introduction

Multidrug-resistant (MDR) Klebsiella pneumoniae is a critical healthcare challenge due to its extensive resistance to antibiotics and role in causing severe infections. Bacteriophages offer a promising alternative for targeting MDR pathogens. This study characterizes a novel phage, vbKpUKJ_2, isolated from hospital sewage water, against clinical K. pneumoniae isolates.

Methods

Phage vbKpUKJ_2 was isolated and purified using the double agar overlay method. Host range and sensitivity were tested against 40 clinical K. pneumoniae isolates using growth inhibition assays. Morphological characterization was performed using transmission electron microscopy (TEM). Genomic analysis was conducted to evaluate the absence of antibiotic resistance genes and determine phyloge

SUBMITTER: Mirza KA 

PROVIDER: S-EPMC11863478 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature