Combining single-cell ATAC and RNA sequencing for supervised cell annotation.

ABSTRACT:

Motivation

Single-cell analysis offers insights into cellular heterogeneity and individual cell function. Cell type annotation is the first and critical step for performing such an analysis. Current methods mostly utilize single-cell RNA sequencing data. Several studies demonstrated improved unsupervised annotation when combining RNA with single-cell ATAC sequencing, but improvements in supervised methods have not been explored.

Results

Single-cell 10x genomics multiome datasets containing paired ATAC and RNA from human peripheral blood mononuclear cells (PBMC) and neuronal cells with Alzheimer's Disease were used for supervised annotation. Using linear and nonlinear dimensionality reduction methods and random forest, support vector machine and logistic regression classifica

SUBMITTER: Gill J 

PROVIDER: S-EPMC11863512 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature