Visualizing spatiotemporal pattern of vascularization by SWIR fluorescence imaging in a mouse model of perforator flap transplantation.

ABSTRACT: Vascularization as a spatiotemporally interlaced process involving angiogenesis and vascular remodeling, has seldom been investigated comprehensively regarding the interrelationship of the two intertwining but sequential processes. Here, a shortwave infrared (SWIR) fluorescence imaging strategy based on quantum dots (QDs) was designed to dynamically visualize vascularization in vivo and in situ in a perforator transplantation mouse model. The vascularization process could be directly perceived from the established flap model with an optimal observation window at 10 min post-injection. Anchored in SWIR technology and image processing, it was revealed that temporally, angiogenesis lasted throughout 21 days after surgery while vascular remodeling took a dominant role after 14 days both in viv

SUBMITTER: Feng S 

PROVIDER: S-EPMC11863596 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature