Regulation of CXCR4 function by S1P₁ through heteromerization.

ABSTRACT:

Background

The trafficking of immune cells between lymphoid organs and circulation depends on gradients of CXCL12 and sphingosine-1-phosphate (S1P), mediated through their cognate receptors C-X-C chemokine receptor type 4 (CXCR4) and S1P receptor type 1 (S1P₁). S1P₁ facilitates the egress of hematopoietic stem cells and lymphocytes by counteracting CXCR4-mediated retention signals. However, the molecular mechanisms underlying this interplay remain poorly understood. In this study, we uncover CXCR4-S1P₁ heteromerization and explore their functional interactions.

Methods

Bimolecular fluorescence complementation (BiFC) assay, proximity ligation assay (PLA), and quantitative bioluminescence resonance energy transfer (BRET) assay were employed to

SUBMITTER: Kim HT 

PROVIDER: S-EPMC11863771 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature