Intermittent fasting regulates gut microbiota and serum metabolome profiles in middle-aged mice fed high-fat diet.

ABSTRACT:

Background

Intermittent fasting (IF) has received wide attention as an effective diet strategy. Existing studies showed that IF is a promising approach for weight control, improving insulin sensitivity and reducing type 2 diabetes mellitus (T2DM) prevalence.

Methods

Twenty-eight 8-month-old male C57BL/6J mice were randomly divided into a normal control group (NC), a high-fat diet group (HFD) and an HFD + IF group. Body weight (BW) and food intake were monitored weekly. After 20 weeks, the intraperitoneal glucose tolerance test (IPGTT), oral glucose tolerance test (OGTT), and intraperitoneal insulin tolerance test (IPITT) were performed weekly in sequence. Fresh faeces were collected to examine changes in gut microbiota, and serum untargeted metabolite profiling was conducted

SUBMITTER: Li Z 

PROVIDER: S-EPMC11863773 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature