Pregnenolone sulfate potentiates tetrodotoxin-resistant Na⁺ channels to increase the excitability of dural afferent neurons in rats.

ABSTRACT:

Background

Although peripheral administration of pregnenolone sulfate (PS) has been reported to produce pronociceptive effects, the mechanisms by which PS modulates the excitability of nociceptive neurons are poorly understood. Here, we report on the excitatory role of PS in peripheral nociceptive neurons, focusing on its effects on tetrodotoxin-resistant (TTX-R) Na⁺ channels.

Methods

TTX-R Na⁺ current (I_Na) mediated by Na_V1.8 was recorded from acutely isolated small-sized dural afferent neurons of rats, identified with the retrograde fluorescent dye DiI, using a whole-cell patch-clamp technique.

Results

Transcripts for enzymes and transporters involved in PS biosynthesis were detected in the ophthalmic branch of the trigemi

SUBMITTER: Jang IS 

PROVIDER: S-EPMC11863801 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature