Potential applications of gene expression profiles obtained from circulating extracellular vesicles in breast cancer.

ABSTRACT:

Background

Liquid biopsy-based biomarkers offer several advantages since they are minimally invasive, can be useful in longitudinal monitoring of the disease and have higher patient compliance. We describe a protocol using minimal volumes of archival and prospective serum/plasma samples to define the RNA contents of EVs and discuss its benefits and limitations.

Methods

RNA-seq analysis of matched tumor biopsy, circulating EVs from breast cancer patients (EV-C, n = 26) and healthy donors (EV-H, n = 4) was performed and differentially expressed genes were validated by RT-PCR in a separate series of samples (EV-C, n = 32 and EV-H, n = 22). A total of 84 samples were studied.

Results

RNA-seq data from 500 μl serum samples yielded more than 17000 genes, of which 320 were D

SUBMITTER: Gupta A 

PROVIDER: S-EPMC11863812 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature