Retinal ganglion cells induce stem cell-derived neuroprotection via IL-12 to SCGF-β crosstalk.

ABSTRACT:

Background

Stem cell-derived secreted factors could protect neurons in neurodegenerative disease or after injury. The exact neuroprotective components in the secretome remain challenging to discover. Here we developed a cell-to-cell interaction model to identify a retinal ganglion cell (RGC)-protective factor derived from induced pluripotent stem cells (iPSCs).

Methods

Primary RGCs were co-cultured with iPSCs or treated with iPSC-conditioned media in vitro. Cell viability were assayed using live-cell staining, and culture supernatant were analyzed via multiplexed antibody-based assays and ELISA. In vivo tests were carried out under mouse optic nerve crush model and RGC transplantation study in rats. Paired t-tests were used for data analysis between two groups.

Results

SUBMITTER: Xia Q 

PROVIDER: S-EPMC11863831 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature