Programming CRISPRi to control the lifecycle of bacteriophage T7.

ABSTRACT: Clustered Regularly Interspaced Short Palindromic Repeats interference (CRISPRi), based on catalytically dead Cas9 nuclease of Streptococcus pyogenes, is a programmable and highly flexible tool to investigate gene function and essentiality in bacteria due to its ability to block transcription elongation at nearly any desired DNA target. In this study, I assess how CRISPRi can be programmed to control the life cycle and infectivity of Escherichia coli bacteriophage T7, a highly virulent and obligatory lytic phage. This is achieved by blocking the expression of critical host-dependent promoters and genes that are required for T7 genome translocation and lifecycle progression. Specifically, I focus on the promoters within the non-coding internalisation signal region and the E

SUBMITTER: Bergmiller T 

PROVIDER: S-EPMC11863960 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature