Rapid and high-sensitivity screening of pregnancy complications by profiling circulating placental extracellular vesicles.

ABSTRACT: Herein, we developed a specific, rapid sensor to quantify placental extracellular vesicle (EV) protein biomarkers of early pregnancy complications. A distinct tetraspanin CD9 and placental alkaline phosphatase (PLAP) expression pattern was observed via targeted multiple reaction monitoring of EVs from maternal plasma collected before 18 weeks of gestation. A classification model was developed using training and validation patient sets, distinguishing between individuals at high risk of developing complications from those with normal pregnancies, achieving 80% sensitivity, 90% specificity, 89% positive predictive value (PPV), and 82% negative predictive value (NPV). Superparamagnetic nanoflowers that captured target EVs (CD9⁺/PLAP⁺) were used to construct a 4-flex glas

SUBMITTER: Palma C 

PROVIDER: S-EPMC11864196 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature