Effect of Omeprazole on Esophageal Microbiota in Dogs Detected Using a Minimally Invasive Sampling Method.

ABSTRACT:

Background

Omeprazole alters the esophageal microbiome (EM) of humans and has associated effects.

Objectives

To assess the changes and subsequent recovery of the EM in awake dogs after omeprazole treatment, using the esophageal string test (EST).

Animals

Ten healthy, client-owned adult dogs.

Methods

A prospective longitudinal design was employed, where esophageal samples were initially collected using EST (day 0), involving the oral administration of an EST capsule and subsequent retrieval after 15 min for pH-based segment identification. The dogs were then administered 1 mg/kg of omeprazole orally, twice daily for 14 days. Follow-up EST samplings were conducted on days 15 and 45. Samples were sequenced targeting the V3-V4 region of the 16S rRNA gene and divers

SUBMITTER: Handa A 

PROVIDER: S-EPMC11864821 | biostudies-literature | 2025 Mar-Apr

REPOSITORIES: biostudies-literature