Modulation of double-negative T cells by Huang-Lian-Jie-Du Decoction attenuates neuroinflammation in ischemic stroke: insights from single-cell transcriptomics.

ABSTRACT:

Introduction

Ischemic stroke (IS) represents a significant global health challenge, characterized by elevated morbidity and mortality rates, largely driven by inflammatory responses. Double-negative T cells (DNTs), a distinct subset of T cells lacking both CD4 and CD8 markers, have been implicated in the pathogenesis of IS, exhibiting potentially dual roles. However, the precise functional contributions of DNTs in this context remain poorly understood.

Methods

In this study, we investigated the role of DNTs during the acute phase of IS and assessed the influence of Huang-Lian-Jie-Du Decoction (HLJD), a traditional Chinese medicinal formula, on these cells. Using single-cell transcriptomics, we identified two distinct subtypes of DNTs: an activated, cytotoxic phenotype (Kill+

SUBMITTER: Wang K 

PROVIDER: S-EPMC11865039 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature