Designing a potent multivalent epitope vaccine candidate against <i>Orientia tsutsugamushi</i> via reverse vaccinology technique - bioinformatics and immunoinformatic approach.

ABSTRACT: Scrub typhus is a life-threatening, undifferentiated febrile illness caused by a gram-negative bacterium, Orientia tsutsugamushi. The bacterial strain is a global health concern that should be considered. Despite several years of effort for the development of an effective immunogenic vaccine, no successful licensed vaccine is available. The aim of the study is to construct an epitope response using a reverse vaccinology approach. The TSA56 and ScaA proteins combined can be the most promising subunit vaccine candidates against O. tsutsugamushi. B-cell, CTL, and HTL epitopes were predicted, and subsequently, all the epitopes were linked by KK, AAY, and GPGPG linkers, respectively, along with an adjuvant at the N-terminal region. Furthermore, molecular docking and MD simulations

SUBMITTER: Panda S 

PROVIDER: S-EPMC11865050 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature