Medroxyprogesterone promotes neuronal survival after cerebral ischemic stroke by inhibiting PARthanatos.

ABSTRACT:

Introduction

Cerebral ischemic stroke (CIS) is caused by the interruption of cerebral blood circulation due to thrombosis or embolism and is the second-leading cause of mortality worldwide. The neuronal death and motor dysfunction resulting from CIS are primarily attributed to the induction of PARthanatos in neurons at the site of ischemia. Blocking parthanatos is a promising treatment for CIS.

Methods

The effect of medroxyprogesterone treatment on PARthanatos in vitro was examined by CCK8 assay and flow cytometry and the target protein of medroxyprogesterone was then identified by a series of assays, including western blotting, immunofluorescence, cell thermal shift assay and molecular docking. Subsequently, the efficacy of medroxyprogesterone in the treatment of ischemic s

SUBMITTER: Yue CL 

PROVIDER: S-EPMC11865058 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature