Deciphering motor dysfunction and microglial activation in mThy1-<i>α</i>-synuclein mice: a comprehensive study of behavioral, gene expression, and methylation changes.

ABSTRACT:

Introduction

Growing recognition of microglia's role in neurodegenerative disorders has accentuated the need to characterize microglia profiles and their influence on pathogenesis. To understand changes observed in the microglial profile during the progression of synucleinopathies, microglial gene expression and DNA methylation were examined in the mThy1-α-synuclein mouse model.

Methods

Disease progression was determined using behavioral tests evaluating locomotor deficits before DNA and RNA extraction at 7 and 10 months from isolated microglia for enzymatic methyl-sequencing and RNA-sequencing.

Results

Pathway analysis of these changes at 7 months indicates a pro-inflammatory profile and changes in terms related to synaptic maintenance. Expression and methylat

SUBMITTER: McGregor BA 

PROVIDER: S-EPMC11865073 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature