Sampling-free investigation of microbial carbon source preferences on renewable feedstocks via online monitoring of oxygen transfer rate.

ABSTRACT: The transition towards sustainable bioprocesses requires renewable feedstocks to reduce dependency on finite resources. While plant-based feedstocks offer significant potential, their complex composition poses new challenges. The microorganisms often exhibit polyauxic growth when presented with multiple carbon sources simultaneously, consuming them in a distinct order according to their carbon source preferences. The traditional investigation of polyauxic growth involves laborious sampling and offline analysis, hindering high-throughput screenings. This study introduces an efficient method for identifying carbon source consumption and their order of metabolization by various microorganisms using the respiration activity monitoring system (RAMOS) in shake flasks. As aerobic carbon metaboliz

SUBMITTER: Grebe LA 

PROVIDER: S-EPMC11865135 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature