Monoclonal antibody generation by controlled immunoglobulin gene rearrangements.

ABSTRACT: Monoclonal antibodies (mAbs) are essential for various applications including experimental reagents, diagnostics, and therapeutics. Thus, the platform technologies that stably generate antigen-specific mAbs are increasingly crucial. We previously developed a method to generate mAbs, termed the "ADLib system", utilizing the avian-derived B cell line DT40. Avian immunoglobulin (Ig) genes diversify principally through gene conversion-a kind of homologous recombination. The ADLib system isolates antigen-specific clones from libraries constructed using DT40 cells treated with Trichostatin A (TSA), a histone deacetylase inhibitor that enhances gene conversion frequencies. The obtained antigen-specific clones are cultured without TSA to minimize further diversification. However, low-frequency spo

SUBMITTER: Murayama A 

PROVIDER: S-EPMC11865429 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature