Targeting pyroptosis reverses KIAA1199-mediated immunotherapy resistance in colorectal cancer.

ABSTRACT:

Background

Despite advancements in treatment modalities, several patients with colorectal cancer (CRC) remain unresponsive to immune checkpoint inhibitor therapy. Pyroptosis, an inflammatory programmed cell death process, holds substantial promise for tumor immunotherapy. In this study, we explored the use of pyroptosis to overcome immunotherapy resistance in CRC.

Methods

We used a pyroptosis-related gene panel to construct an immunotherapy efficacy evaluation model and validated its performance by immunohistochemical staining of CRC patient samples. Pyroptosis and its underlying mechanisms were examined both in vitro and in vivo using PCR, western blotting, lactate dehydrogenase release assay, ELISA, co-immunoprecipitation, immunohistochemistry, fluorescence cell assays, mi

SUBMITTER: Li L 

PROVIDER: S-EPMC11865760 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature