Thermostability and Activity Improvements of PETase from Ideonella sakaiensis.

ABSTRACT: Polyethylene terephthalate (PET), a widely used plastic, is a significant environmental pollutant due to its persistence. While the PET-degrading enzyme PETase from Ideonella sakaiensis offers promising solutions, its limited activity at higher temperatures hinders its practical application. This study aimed to enhance the PETase performance through protein engineering. We introduced multiple amino acid substitutions to the wild-type I. sakaiensis PETase to improve its thermostability, substrate binding, and catalytic activity. Several potential mutant IsPETases were generated using computational design and evaluated in silico. The selected mutant was then produced in E. coli BL21(DE3). Finally, the catalytic activity of the purified mutant IsPETase was examined

SUBMITTER: Stevensen J 

PROVIDER: S-EPMC11866010 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature