Surface Engineering of the Encapsulin Nanocompartment of Myxococcus xanthus for Cell-Targeted Protein Delivery.

ABSTRACT: Encapsulin nanocompartments (ENCs), or simply encapsulins, are a novel type of protein nanocage found in bacteria and archaea. The complete encapsulin systems include protein cargoes involved in specific metabolic tasks. Cargoes are selectively encapsulated due to the presence of a specific cargo-loading peptide (CLP). However, heterologous proteins fused to the CLP have also been successfully encapsulated, making encapsulins a very promising system for protein-carrying and delivery. Nevertheless, for precise cell or tissue delivery, encapsulins require the addition of tagging peptides or proteins. In this study, the external surface of the Myxococcus xanthus ENC (MxENC) was analyzed and modified to carry the bioorthogonal conjugation peptide (SpyTag) to further decorate the MxENCs

SUBMITTER: Gomez-Barrera SN 

PROVIDER: S-EPMC11866011 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature