Ergothioneine Stimulates Ca²⁺-Mediated Brain-Derived Neurotrophic Factor Expression in NE-4C Nerve Cells.

ABSTRACT: Ergothioneine (EGT), a naturally occurring histidine derivative, has been reported to modulate neurodegenerative diseases; however, the underlying mechanism remains unclear. This study aimed to investigate the brain-beneficial role of the natural amino acid EGT in NE-4C nerve cells. In the nerve cells, EGT treatment of >10 μM for 48 h significantly increased the expression of brain-derived neurotrophic factor (BDNF), as well as the phosphorylation of cAMP response element-binding protein (CREB), whereas no change was observed in acetylcholine receptor expression. Additionally, EGT induced an increase in intracellular Ca²⁺ levels via stimulation of the inositol 1,4,5-triphosphate receptor (IP₃R) in the endoplasmic reticulum; this increase was abrogated by the inhibitio

SUBMITTER: Shi C 

PROVIDER: S-EPMC11866013 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature