Urinary IR700 Ligand as an Early Biomarker of Therapeutic Efficacy of Near-Infrared Photoimmunotherapy.

ABSTRACT: Near-infrared photoimmunotherapy (NIR-PIT) is a cell-selective cancer therapy employing monoclonal antibody-photoabsorber conjugates (APCs) and near-infrared (NIR) light. When exposed to NIR light, the photoabsorber, IR700, releases an axial ligand, resulting in a transition of the remaining molecule from water-soluble to hydrophobic. This results in the death of APC-bound cells by physical damage to the cell membrane. The amount of released IR700 ligand reflects the completeness of the photochemical reaction and, therefore, can be a biomarker for treatment efficacy. In this study, we developed and validated a quantitative analytic method for detecting excreted IR700 ligands in urine using a liquid chromatograph-triple quadrupole mass spectrometer (LC-MS/MS). In A431 tumor-bearing mice tre

SUBMITTER: Okuyama S 

PROVIDER: S-EPMC11866193 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature