An Alternative In Vitro Methodology for the Quality Control of Equine-Chorionic Gonadotropin in Commercial Products.

ABSTRACT: Equine-chorionic gonadotropin (eCG) is widely used in fixed-time artificial ovulation and superovulation protocols to improve reproductive performance. Commercial products available in the market for veterinary use consist of partially purified preparations of pregnant mare serum gonadotropin (PMSG). eCG is a heterodimeric glycoprotein and is thus extremely challenging to quantify through chromatography. Pharmaceutical companies usually carry out in vivo methods to measure the potency of finished products containing eCG. Considering the three Rs principle (refinement, replacement, and reduction), the aim of this study was to develop in vitro assays using high-performance liquid chromatography (HPLC) and cell culture techniques to predict the biological activity of products co

SUBMITTER: Perez MM 

PROVIDER: S-EPMC11866194 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature