Rapid, Economical Detection of Helicobacter pylori Using Gold Colloidal Nanoparticle Biosensors.

ABSTRACT: Nucleic acid tests (NAT), the gold standard diagnostic technology, play a crucial role in the prevention of infectious diseases. However, PCR, the current state-of-the-art NAT, is expensive, slow, and requires dedicated infrastructure and facilities. Therefore, there exists an urgent need to create alternative molecular diagnostic technologies. We describe the use of a gold colloidal nanobiosensor detection system that can specifically and sensitively detect the 16S rRNA gene of the worldwide gastric pathogen Helicobacter pylori. We demonstrate the systematic identification of oligonucleotide probe sequences according to secondary structure, binding energy, and homology search criteria. We selected three probe sequences that were used to evaluate the detection of a 120 nt synthetic

SUBMITTER: Jiang J 

PROVIDER: S-EPMC11866199 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature