Single-Probe Sensing Array Based on Au Nanozyme for Simple, Rapid, and Low-Cost Colorimetric Identification of Antioxidants.

ABSTRACT: Identification of biological antioxidants is of vital importance because of the essential role of antioxidants in keeping the balance of various diseases. Here, we designed a simple, rapid, and low-cost nanozyme sensing array for colorimetric identification of multiple antioxidants based on Au nanoparticles (Au NPs) synthesized via a facile and green aqueous phase method. In the presence of H₂O₂, Au NPs possessed peroxidase-like catalytic activity and could effectively catalyze the color-less 3,3',5,5'-tetramethylbenzidine (TMB) to blue oxidized product with comparable enzyme kinetics parameters. The function of the colorimetric sensing array was based on the inhibitory effect of diverse antioxidants on the chromogenic system to varying degrees under different pH cond

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11866201 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature