Electrochemical Paper-Based Analytical Device (e-PAD) Using Immobilized Prussian Blue and Antibodies for the Diagnosis of Leishmania.

ABSTRACT: Leishmaniasis is a neglected disease prevalent in remote and economically disadvantaged regions. Its diagnosis can be achieved through various methods, with electroanalysis emerging as an excellent alternative for antigen detection due to its simplicity, sensitivity, and cost-effectiveness. Herein, a qualitative electrochemical paper-based analytical device (e-PAD) using unmodified screen-printed electrodes for the immunoassay of Leishmania amazonensis antigens has been developed. The detection is based on a sandwich-type assembly, utilizing two biological elements, one for capture and one for detection, with the target antigen sandwiched between them. Antibodies against Leishmania amazonensis and Prussian blue, serving as a redox mediator, were immobilized on the paper subst

SUBMITTER: Papi MAP 

PROVIDER: S-EPMC11866208 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature