Molecular Profiling of Glioblastoma Patient-Derived Single Cells Using Combined MALDI-MSI and MALDI-IHC.

ABSTRACT: In recent years, mass spectrometry-based imaging techniques have improved at unprecedented speeds, particularly in spatial resolution, and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) experiments can now routinely image molecular profiles of single cells in an untargeted fashion. With the introduction of MALDI-immunohistochemistry (IHC), multiplexed visualization of targeted proteins in their native tissue location has become accessible and joins the suite of multimodal imaging techniques that help unravel molecular complexities. However, MALDI-IHC has not been validated for use with cell cultures at single-cell level. Here, we introduce a workflow for combining MALDI-MSI and MALDI-IHC on single, isolated cells. Patient-derived cells from glioblastoma

SUBMITTER: Krestensen KK 

PROVIDER: S-EPMC11866282 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature