Single-cell analysis of cerebrospinal fluid reveals common features of neuroinflammation.

ABSTRACT: Neuroinflammation is often characterized by immune cell infiltrates in the cerebrospinal fluid (CSF). Here, we apply single-cell RNA sequencing to explore the functional characteristics of these cells in patients with various inflammatory, infectious, and non-inflammatory neurological disorders. We show that CSF is distinct from the peripheral blood in terms of both cellular composition and gene expression. We report that the cellular and transcriptional landscape of CSF is altered in neuroinflammation but is strikingly similar across different neuroinflammatory disorders. We find clonal expansion of CSF lymphocytes in all disorders but most pronounced in inflammatory diseases, and we functionally characterize the transcriptional features of these cells. Finally, we explore the genetic con

SUBMITTER: Jacobs BM 

PROVIDER: S-EPMC11866449 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature