Binding affinity and transport studies of engineered photocrosslinkable affibody-enzyme-nanoparticle constructs.

ABSTRACT: Nanoparticle accumulation at tumor sites has been well reported in vivo, where targeting typically shows increased retention, but challenges remain for clinical translation. This work examines the effect of targeting ligand binding affinities and nanoparticle size on retention and transport through a solid tumor. We first show using cell affinity assays that modifying a wildtype (WT) anti-epidermal growth factor receptor (EGFR) affibody-enzyme fusion protein into a UV-photocrosslinkable (N23BP) version led to a significant decrease in affinity, whether as a free protein or as a conjugate to silica nanoparticles. Despite the reduced EGFR affinity, all protein conjugated nanoparticles showed binding and uptake to EGFR-overexpressing HTB9 bladder cancer cells as detected by confocal mi

SUBMITTER: Curry SD 

PROVIDER: S-EPMC11866575 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature