A proinflammatory response and polarized differentiation of stromal elements characterizes the murine myeloma bone marrow niche.

ABSTRACT:

Background

The bone marrow (BM) niche contains non-hematopoietic elements including mesenchymal stromal cells (MSC) and bone marrow endothelial cells (BMEC) which provide mechanical support, and control hematopoietic cell growth and differentiation. Although it is known that multiple myeloma (MM) cells interact closely with the BM microenvironment, little is known about the impact of MM on non-hematopoietic niche-forming cells.

Methods

To address the role of the niche in MM pathogenesis, we utilized the 5TGM1 murine model. During the asymptomatic precursor stage of the model, we isolated the rare non-hematopoietic cells and performed single cell RNA sequencing. Using in-silico methods we characterized the individual cellular components of the niche, their relative abundance

SUBMITTER: Ghamlouch H 

PROVIDER: S-EPMC11866767 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature