Akt2 inhibition alleviates temporomandibular joint osteoarthritis by preventing subchondral bone loss.

ABSTRACT:

Background

This study aimed to investigate the role and mechanism of the Akt2 pathway in different stages of anterior disc displacement (ADD)-induced temporomandibular joint osteoarthritis (TMJOA).

Methods

A rat model for TMJOA that simulates anterior disc displacement was established. For inhibit Akt2 expression in subchondral bone, rats were intravenously injected with adeno-associated virus carrying Akt2 shRNA at a titer of 1 × 10¹² transducing units/mL 10 days before the ADD or sham operations. The rats were euthanized and evaluated 1 or 8 weeks after surgery, as these time points represented the early or advanced stage of ADD. Immunostaining was performed to examine the expression and location of phosphorylated Akt2 in different stages of ADD. Microcomputed t

SUBMITTER: Feng SY 

PROVIDER: S-EPMC11866854 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature