Radiotherapy resistance driven by Asparagine endopeptidase through ATR pathway modulation in breast cancer.

ABSTRACT:

Background

Tumor resistance represents a major challenge in the current oncology landscape. Asparagine endopeptidase (AEP) overexpression correlates with worse prognosis and reduced overall survival in most human solid tumors. However, the underlying mechanisms of the connection between AEP and reduced overall survival in cancer patients remain unclear.

Methods

High-throughput proteomics, cellular and molecular biology approaches and clinical data from breast cancer (BC) patients were used to identify novel, biologically relevant AEP targets. Immunoblotting and qPCR analyses were used to quantify protein and mRNA levels. Flow cytometry, confocal microscopy, chemical inhibitors, siRNA- and shRNA-silencing and DNA repair assays were used as functional assays. In-silico analyse

SUBMITTER: Morillo-Huesca M 

PROVIDER: S-EPMC11866873 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature