Selective Quantification of Charged and Neutral Polyfluoroalkyl Substances Using the Total Oxidizable Precursor (TOP) Assay.

ABSTRACT: Perfluoroalkyl acid (PFAA) precursors are a diverse subclass of per- and polyfluoroalkyl substances (PFASs) that can be transformed into PFAAs of public health concern. Unlike strongly acidic PFAAs, precursors can be anionic, cationic, neutral, or zwitterionic. Precursor charge affects the environmental fate, but existing quantification techniques struggle to ascertain the abundance of compounds within each charge group. To fill this gap, we developed and validated a solid-phase extraction procedure that separates precursors by charge and quantifies the sum of the precursors in each fraction with the total oxidizable precursor (TOP) assay. Method performance was demonstrated by spiking known concentrations of ten precursors into aqueous film-forming foam (AFFF)-impacted groundwater, munici

SUBMITTER: Antell EH 

PROVIDER: S-EPMC11866920 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature