Memory CD4+ T cells sequentially restructure their 3D genome during stepwise activation.

ABSTRACT:

Background

CD4+ T cells are a highly differentiated cell type that maintain enough transcriptomic plasticity to cycle between activated and memory statuses. How the 1D chromatin state and 3D chromatin architecture support this plasticity is under intensive investigation.

Methods

Here, we wished to test a commercially available in situ Hi-C kit (Arima Genomics Inc.) to establish whether published performance on limiting cell numbers from clonal cell lines copies across to a primary immune cell type. We achieved comparable contact matrices from 50,000, 250,000, and 1,000,000 memory CD4+ T-cell inputs. We generated multiple Hi-C and RNA-seq libraries from the same biological blood donors under three separate conditions: unstimulated fresh ex vivo, IL-2-only stimul

SUBMITTER: Ward AI 

PROVIDER: S-EPMC11866950 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature