Combining Quantitative Proteomics and Interactomics for a Deeper Insight into Molecular Differences between Human Cell Lines.

ABSTRACT: In modern biomedical research, cultivable cell lines are an indispensable tool, and the selection of cell lines that exhibit specific functional profiles is often critical to success. Cellular functional pathways have evolved through the selection of protein intra- and intermolecular interactions collectively referred to as the interactome. In the present work, quantitative in vivo protein cross-linking and mass spectrometry were used to probe large-scale protein interactome differences among three commonly employed human cell lines, namely, HEK293, MCF-7, and HeLa cells. These data illustrated highly reproducible quantitative interactome levels with R² values larger than 0.8 for all biological replicates. Proteome abundance levels were also measured using data-independen

SUBMITTER: Bakhtina AA 

PROVIDER: S-EPMC11867029 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature