Finding the best column for polar basic analytes across reversed-phase and hydrophilic interaction liquid chromatography.

ABSTRACT:

Background

The chromatographic measurement of polar molecules is often surprisingly complicated. On the one hand, the standard experiments use C18 columns, which is usually unsuitable. On the other hand, these types of molecules can behave vastly differently despite their similarities. Thus, finding the right chromatographic conditions is challenging. HILIC can be an obvious choice, but C18 is still used with suitable analytes. Comparing the two methods would be advantageous, but a numerical comparison can be problematic. Thus, a simple comparing and ranking system is needed and put to use to find the best method for the separation of polar basic molecules.

Results

In this paper, nine columns with different stationary phases, from reversed-phase to hydrophilic interaction, a

SUBMITTER: Simon J 

PROVIDER: S-EPMC11867270 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature