High Concentration Hydrogen Protects Sepsis-Associated Encephalopathy by Enhancing Pink1/Parkin-Mediated Mitophagy and Inhibiting cGAS-STING-IRF3 Pathway.

ABSTRACT:

Background

Sepsis-associated encephalopathy (SAE) leads to increased mortality. Hydrogen (H₂) has been proven to be effective in protecting against SAE. This study aimed to investigate the protective mechanism of a high concentration of H₂ (HCH) (67%) against SAE.

Methods

A mouse sepsis model was established via cecal ligation and puncture (CLP). 67% H₂ was inhaled for 1 h at 1 h and 6 h after the operation. First, mice were randomly divided into 5 groups: Sham, CLP, CLP + CQ (a mitophagy inhibitor), CLP + H₂, and CLP + H₂ + CQ. Seven-day survival, cognitive function, and hippocampal damage were assessed. Then, mice were randomly divided into four groups: Sham, CLP, CLP + UA (a mitophagy agonist), and CLP + H₂

SUBMITTER: Cui Y 

PROVIDER: S-EPMC11867788 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature