Enzyme immobilization with plant-based polysaccharides through complex coacervation.

ABSTRACT: Plant-based polysaccharides (PSs) were used to immobilize phytase in a coacervate system. Molecular docking predicted the intermolecular interactions and conformations between the phytase and the polysaccharide and correlated them to the activity recovery of phytase in the coacervate complex. PSs with two different functional groups, sulfate (iota (IC), lambda (LC), and kappa (KC) carrageenan) and carboxylate (low methoxyl pectin (LMP) and sodium alginate (SA)) were investigated. The optimized conditions for coacervation and activity recovery were pH 4 with a phytase-to-polysaccharide volume ratio of 12:1. Zeta potential measurements, FTIR spectroscopy, and molecular docking confirmed that electrostatic interactions and hydrogen bonding were the main driving forces for coacervate formation

SUBMITTER: Khongkomolsakul W 

PROVIDER: S-EPMC11867993 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature