Higher Reactive Oxygen Species and cellular aging in oral mucosal cells of young smokers: a comparative analytical study.

ABSTRACT:

Background

Cigarette smoke contains chemical components that cause excessive production of Reactive Oxygen Species (ROS), altering cell physiology and affecting key pathways. This leads to hyperinflammation, DNA damage, and cellular aging, which may cause oral and pulmonary pathologies. Our study aims to investigate the impact of smoking on ROS levels and cellular aging in oral mucosal cells. We compared Reactive oxygen Species and cellular aging between smokers and non-smokers. Secondarily, we also compared the results between young and old smokers.

Methods

Oral swabs were taken from 50 smokers and 50 nonsmokers using a cytology brush. We quantified the reactive oxygen species (ROS) by using oxidized 2'7' dichlorodihydrofluorescein-diacetate (DCFH-DA) dye. To assess cellula

SUBMITTER: Imdad B 

PROVIDER: S-EPMC11868274 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature