Integrative single-cell and spatial transcriptome analysis reveals heterogeneity of human liver progenitor cells.

ABSTRACT:

Background

Liver progenitor cells (LPCs) with bipotential differentiation capacities are essential for restoring liver homeostasis and hepatocyte population after damage. However, the low proportion and shared markers with epithelial cells make studying LPC heterogeneity difficult, especially in humans. To address this gap, we explored over 259,400 human liver single cells across 4 conditions (fetal, healthy, cirrhotic, and HCC-affected livers).

Methods

Human liver tissue samples were analyzed using spatial transcriptomics sequencing technologies to describe the heterogeneity of LPCs. Liver tissue was characterized by LPC heterogeneity at single-cell resolution by employing cellular modules, differentiation trajectories, and gene co-expression patterns.

Results

We ann

SUBMITTER: Liu C 

PROVIDER: S-EPMC11868439 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature