µMap proximity labeling in living cells reveals stress granule disassembly mechanisms.

ABSTRACT: Phase-separated condensates are membrane-less intracellular structures comprising dynamic protein interactions that organize essential biological processes. Understanding the composition and dynamics of these organelles advances our knowledge of cellular behaviors and disease pathologies related to granule dysregulation. In this study, we apply microenvironment mapping with a HaloTag-based platform (HaloMap) to characterize intracellular stress granule dynamics in living cells. After validating the robustness and sensitivity of this approach, we then profile the stress granule proteome throughout the formation and disassembly and under pharmacological perturbation. These experiments reveal several ubiquitin-related modulators, including the HECT (homologous to E6AP C terminus) E3 ligases I

SUBMITTER: Pan CR 

PROVIDER: S-EPMC11868469 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature