Loss of hepatocyte identity following aberrant YAP activation: A key mechanism in alcoholic hepatitis.

ABSTRACT:

Background & aims

Alcoholic hepatitis (AH) is a life-threatening disease with limited therapeutic options, as the molecular mechanisms leading to death are not well understood. This study evaluates the Hippo/Yes-associated protein (YAP) pathway which has been shown to play a role in liver regeneration.

Method

The Hippo/YAP pathway was dissected in explants of patients transplanted for AH or alcohol-related cirrhosis and in control livers, using RNA-seq, real-time PCR, western blot, immunohistochemistry and transcriptome analysis after laser microdissection. We transfected primary human hepatocytes with constitutively active YAP (YAPS127A) and treated HepaRG cells and primary hepatocytes isolated from AH livers with a YAP inhibitor. We also used mouse models of ethanol exposu

SUBMITTER: Bou Saleh M 

PROVIDER: S-EPMC11868489 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature