Commercial immunoassays in paraneoplastic neurological syndromes: an Australian laboratory perspective.

ABSTRACT:

Background

Paraneoplastic antibodies are implicated in heterogeneous clinical presentations. Commercial immunoassays include indirect immunofluorescence (IIF), and line immunoblot (LIB). LIB can be associated with false positives, and unfortunately, further confirmatory assays are not readily available in diagnostic laboratories.

Objectives

To determine frequency of positive LIB on serum or cerebrospinal fluid (CSF) using EUROLine paraneoplastic neurological syndromes (PNS) 12 Ag Test kit (EuroImmun, Germany) and establish concordance with IIF on Nova Lite kit (Inova Diagnostics, United States) and clinical presentation.

Methods

A retrospective analysis of all LIB performed over a four-year period was undertaken. Healthy control samples were also analysed with IIF and

SUBMITTER: Ali SB 

PROVIDER: S-EPMC11868762 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature